Tutorial

Overview

Before you begin, please make sure that

• MUST is correctly installed;
• The path has been added to the enviroment variable ${PATH}. (Otherwise, you may run MUST with ${ROOTPATH}/must.)

Tutorial-1 Tomogram reconstruction with MUST

This section shows how to use MUST to reconstruct a tomogram from a cryo-ET tilt-series.

• Step 0: Get the tutorial dataset

Download the tilt-series and the parameter file for this tutorial from here.

Decompress the zip file and enter the folder.

• Step 1.1: Reconstruct with WBP-like filter

must para_wbp.conf

• Step 1.2: Reconstruct with SIRT-like filter

must para_sirt.conf

• Step 1.3: Reconstruct with CTF deconvolution

must para_ctf.conf

• Step 1.4: Reconstruct with 3D-CTF correction

must para_3dctf.conf

• Step 2: Visualize the reconstructed tomogram

• Note 1: CTF deconvolution shows better constrast for visualization than 3D-CTF correction, because 3D-CTF correction multiplies another CTF term to the tomogram. However, 3D-CTF-corrected tomograms may lead to higher-resolution structures for subsequent subtomogram averaging.

• Note 2: If you try to reconstruct your own data of a larger size (e.g., an unbinned tomogram), it will take more time and require more GPU memory. If you run out of GPU memory, you can try to increase the parameter "N_block_rec" and "N_block_linear_combination" to divide the reconstruction process into more blocks.